FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16742603
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC618130.36919587712854446No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT315880.1886683928419016No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT209860.12534490604597148No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT204060.12188068963947839No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT202670.1210504722593016TruSeq Adapter, Index 15 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA183520.10961258533096677No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT181060.10814327975166108No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA175480.10481046465713843No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA172970.10331129514329404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1088650.0113.9865341
CGGGCGC297750.0113.9498141
CGGGTGC89000.0112.681711
CGGGTAC115600.0109.753631
CGGCTAA6300.0104.80171
GGGCGCG351200.0103.0875242
CGGATAC14000.096.870761
GGCGCGG364450.096.121133
CGCGGTG364750.095.50355
CGGGTAT322600.094.6070561
GCGCGGT361850.094.494344
CGGAATA158350.093.646181
CGGGTGT288700.093.436461
CGGAAAT92350.091.976561
CGGGCGT417850.091.788971
CGGGTTT804450.091.583631
CGGATGC11350.090.664251
CGGTAAT111800.090.1275251
CGGTGGT645050.089.418097
CGGGTAA75350.088.57181
Produced by FastQC (version 0.11.5)