FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22899338
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC756680.330437499983624No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT448650.19592269435911203No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA427830.18683072846909374No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA415230.1813283859996302No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT392250.171293161400561No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA382210.1669087551788615No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG360940.15762027705779091No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT329790.14401726373050608No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA298410.13031381081846122No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT270930.11831346390886933No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA263570.11509939719654778No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT258820.1130251014243294No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA252100.11009051877394883No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT229610.10026927415980322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC368100.0114.203081
CGGTTAA1370550.0113.887271
CGGGTGC117650.0113.208831
CGGGTAC139500.0108.945891
CGGCTAA11950.0104.490221
GGGCGCG436050.0102.533982
CGGAATA261350.096.510021
CGCGGTG452400.095.7437065
GGCGCGG453050.095.6061253
GCGCGGT451450.094.233284
CGGGTAT433650.093.087361
CGGGTGT385350.091.8554151
CGGGCGT569750.091.556261
CGGGTTT1026900.090.29291
CGGAATT266050.088.994311
GCGGTGG659750.088.522286
CGGTAAT142850.088.242911
CGGTGGT814650.088.1243067
CGGATAA106250.086.797481
CGGATAC17900.086.6985241
Produced by FastQC (version 0.11.5)