FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18804806
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC619260.32930943291837206No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT346550.18428799531353846No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC320690.17053619165228293TruSeq Adapter, Index 5 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA256320.13630558060529846No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT206450.10978576434130723No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA193740.10302685387980073No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA193540.10292049808969049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC302650.0114.214651
CGGTTAA1225650.0114.003931
CGGGTGC87300.0113.911121
CGGCTAA8800.0109.753051
CGGGTAC108700.0107.0069661
GGGCGCG357000.0103.58562
GGCGCGG371400.096.760593
CGCGGTG372950.095.8958055
GCGCGGT368650.095.464964
CGGGCGT446000.092.8504941
CGGAATA162850.092.659231
CGGGTGT276000.091.847741
CGGGTAT325800.091.7890241
CGGGTTT810450.091.239911
CGGTAAT104600.090.682361
CGGATGC13250.090.440821
CGGAATT205700.090.3703841
CGGTGGT641300.090.151817
GCGGTGG530550.089.7602846
CGGATAA86150.089.618731
Produced by FastQC (version 0.11.5)