FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23246490
Sequences flagged as poor quality0
Sequence length125
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT594440.2557117225009023No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA469240.20185412937609076No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC461730.19862353413354017No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA384700.16548734884277153No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT363520.15637629594833458No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT238020.10238965108280862No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA236810.10186914239526054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC98650.0109.2802661
CGGTTAA755400.0107.668081
CGGGCGC251500.0104.083451
CGGCTAA6600.0103.879071
CGGGTAC104850.0100.563491
GGGAGGC601100.095.54232
CGGGAGG1412800.094.135711
CGGGTGT334300.093.2433241
CGGTAAT147050.089.509511
CGGGTAT375000.089.270091
CGGAATA282250.089.037571
GGGCGCG312650.088.835692
CGGGTTT759150.087.898271
CGGGTAA112600.087.343161
CGGGAAG212950.086.955431
GCGGAGT386050.086.3679667
CGGAGTG126850.083.588381
CGGTATT355700.083.097311
CGGGCGT465600.082.816141
CGGGATA109100.082.615021
Produced by FastQC (version 0.11.5)