FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8113346
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC371500.45788753493318296No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC361430.44547588627429424TruSeq Adapter, Index 6 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT164550.20281398081629948No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA125100.15419039197884574No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA117610.14495868905381332No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT102270.12605156984553598No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT93210.11488478366385459No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT90320.11132275142709308No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA81680.10067363082999295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC186500.0114.4813461
CGGGTGC54800.0113.274661
CGGTTAA392200.0109.7883151
CGGGTAC73450.0106.086841
GGGCGCG213300.0103.942992
CGCGGTG219950.097.530855
GCGCGGT217050.097.189474
CGGCTAA3750.096.904551
GGCGCGG222600.096.637023
CGGGTAT202300.096.617531
CGGGTGT172500.096.1102451
CGGGCGT265550.094.0865861
CGGGTTT382150.091.381341
CGGTGGT386150.089.967647
GCGGTGG314350.089.776116
GGGAGGC219200.089.659512
CGGTAAT54850.089.060031
CGGGAGG541700.087.846571
CGGAATA82400.087.840461
CGGGTAA46700.086.87121
Produced by FastQC (version 0.11.5)