FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14969827
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC522040.34872814495451415No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA336760.22495917955498082No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT330070.22049019003359227No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT267700.1788263818947273No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG228460.15261365411904892No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA226590.151364474686314No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT196180.1310502786705551No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA196010.13093671690394285No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA186330.12447037631096204No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA184160.12302079376067605No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT176100.11763663000247099No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC175650.11733602532614437TruSeq Adapter, Index 4 (100% over 50bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT171940.11485770677242962No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT166310.11109680826638811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC260400.0113.8816451
CGGGTGC86300.0112.1941
CGGTTAA691950.0112.03741
CGGGTAC102150.0110.1262051
CGGCTAA5150.0107.597651
GGGCGCG316250.099.199192
CGGGTAT298500.096.032461
CGGAATA169350.095.2423551
CGGGTGT276750.094.149691
CGCGGTG323150.093.258185
CGGATGC9750.092.278261
GGCGCGG329300.091.950043
CGGGCGT401600.091.37841
CGGTAAT96600.091.164211
GCGCGGT325600.091.05834
CGGGTTT614400.089.879751
GGGAGGC322050.087.8544162
CGGATAC12700.087.73341
CGGGAGG833750.087.6299441
CGGGTAA73200.087.58471
Produced by FastQC (version 0.11.5)