FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10361892
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT571250.5512989326659649No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC414030.3995698854996751No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA391660.37798116405768367No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT321930.3106865039705104No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA260160.25107383863873506No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA166870.1610420181951327No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC166080.160279609167901TruSeq Adapter, Index 12 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA164140.15840736421495225No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT161070.15544458483064677No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT158690.1531477070017715No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT127250.12280575786738561No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA123570.11925428290509107No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT122670.11838571565887775No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT117250.11315501068723743No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA113630.10966144020802379No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA113210.10925610882645757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC195800.0113.1371841
CGGGTGC61850.0112.966631
CGGTTAA469600.0111.925561
CGGGTAC75100.0109.533161
GGGCGCG229700.0101.088282
CGGGTAT223300.097.576861
CGGGTGT189600.097.4530261
GGGAGGC309950.095.093582
CGCGGTG235900.095.045215
CGGCTAA4650.094.791771
CGGAATA187350.094.712791
GGCGCGG238400.094.348033
GCGCGGT233350.093.967354
CGGGAGG739900.093.336641
CGGGTTT404950.092.447631
CGGTAAT67600.091.2861561
CGGGTAA55900.091.212391
CGGGCGT297100.090.279921
CGGTGGT421050.088.055647
GCGGTGG342350.087.722796
Produced by FastQC (version 0.11.5)