FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568511
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGNGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC404650.17929849248805116No Hit
TGNAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA302360.13397427947284604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC253900.0208.560871
CGGGTGC80950.0204.293521
CGGCTAA5200.0192.745471
CGGTTAA502800.0191.896991
CGGGTAC100500.0189.706711
GGGCGCG315300.0179.080022
GGCGCGG336250.0161.045563
CGGGTAT326400.0159.50581
CGGGTGT266850.0157.082371
CGGGCGT407800.0153.582981
GGGAGGC452650.0149.251822
CGGATAC11800.0149.114561
CGGGAGG1030500.0146.205171
CGGGTTT519200.0140.427661
CGGGTAA83700.0140.103151
CGGAATA122650.0140.01091
CGGTAAT101400.0139.479611
CGGATGC9400.0131.504351
CGGAATT153650.0129.375091
CGGAAAT77200.0127.664741
Produced by FastQC (version 0.11.5)