FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20520144
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC530400.25847771828501787No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA287660.14018420143640317No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT264110.1287076737863048No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG257630.12554980120997203No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA209980.10232871660159891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1137200.0111.9632951
CGGGCGC321650.0109.785231
CGGGTGC105000.0108.532411
CGGGTAC128900.0106.044481
GGGCGCG377250.098.502642
CGCGGTG382700.092.659355
CGGGTAT418850.092.4490051
GGCGCGG390700.092.088953
GCGCGGT384900.091.327834
CGGGCGT542900.090.802991
CGGGTTT1160600.090.385841
CGGGTGT373450.090.238951
CGGAATA189250.089.490921
CGGATGA82800.087.681791
CGGAATT247450.086.912181
CGGAAAT112550.086.500331
CGGTAAT128400.086.3431241
CGGATAA89250.085.812451
CGGTGGT696250.084.849037
CGGAGTG123500.084.757641
Produced by FastQC (version 0.11.5)