FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113935
Sequences flagged as poor quality0
Sequence length125
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA191640.10579700103815103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC62350.0106.548591
CGGCTAA5100.0101.729771
CGGTTAA362550.0100.781071
CGGGCGC122100.0100.465651
CGGGTAC54750.098.029631
CGGGCTT5600.097.971281
CGGGTAA91150.095.323911
GGGAGGC341950.094.6852952
GGGTGGC80050.092.326562
CGGGGAC24800.091.856661
CGGGTGT223650.091.165221
CGGGAGG777450.090.4051061
TCCGATC2000.089.2466058
CGGAATA184000.088.576971
GCGGAGT272050.088.530367
CGGGCAT3850.088.290321
CTTCCGA2100.087.833146
CCGATCT2100.084.996769
CGGGTTT501450.084.959661
CGGATTG92850.084.45841
Produced by FastQC (version 0.11.5)