FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14333136
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC572920.39971713098933825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT550120.3838099352437596TruSeq Adapter, Index 18 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT181870.12688779343194678No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT180430.1258831284374892No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT171450.11961792590260778No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA150290.10485493195627252No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT149550.10433864577856512No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA146730.10237117683108568No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA143580.10017347215570968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC98450.0113.5085751
CGGGCGC310300.0113.395751
CGGTTAA661750.0110.836161
CGGGTAC123750.0108.978991
CGGCTAA5350.0101.320931
GGGCGCG364900.0101.2719962
CGGGTAT336600.096.607431
CGGGTGT292200.095.222861
CGCGGTG372550.095.146685
GGCGCGG378550.094.754673
GCGCGGT371300.094.2490164
CGGGCGT425250.093.739611
CGGATGC9950.091.596721
CGGTAAT94050.091.5209661
CGGGTTT599750.089.796161
CGGTGGT671400.089.078897
CGGGAGG854300.088.246531
GCGGTGG545050.088.217256
GGGAGGC348250.088.2031862
CGGAATA114200.087.265261
Produced by FastQC (version 0.11.5)