FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17500767
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC359450.2053909980059731No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT254110.14519935040561363No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT245740.14041670287936522No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA245330.14018242743303763No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA191260.10928663869417837No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA187600.107195301783059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC89350.0113.195051
CGGGCGC213100.0112.38451
CGGGCAT4350.0111.043181
CGGTTAA494350.0108.4358751
CGGGTAC78250.0105.017341
CGGGGAC26800.0100.5772551
CGGGCTT6150.098.905671
GGGCGCG266150.098.5549242
CGGCTAA4350.097.334151
CGGGTAA114250.097.137371
CGGGTGT282700.096.929481
CGGGCTA1850.096.704271
CGGTCTA1000.095.414891
GGGAGGC407350.095.215972
CGGGCAC1350.092.7644651
CGGGAGG884400.091.73731
CGGGTAT262850.091.204071
CGGGTTT548300.090.207351
GGCGCGG280650.090.1336063
CGGTACT600.089.4514541
Produced by FastQC (version 0.11.5)