FastQCFastQC Report
Thu 12 May 2022
EGAF00005418742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28648233
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC767970.2680688892749511No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT740840.2585988462185434No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA640580.22360192337167883No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA610840.21322082936144787No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT494060.17245740775705085No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT472000.16475710735806987No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT446730.15593631900438676No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT436750.15245268355643438No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA430430.15024661381384324No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT411750.14372614185314675No Hit
TGGTTTTATGAAAGGAAGTGTTTAATTTTATTGAGTTGAATGTAAATATT357840.12490822732417738No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA325860.11374523517733187No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT301320.10517926184138476No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT298230.10410066128685841TruSeq Adapter, Index 14 (97% over 44bp)
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA287550.10037268267121396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)