FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005418743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19416475
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC587480.302567793587662No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT435080.22407774840695854No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT374800.19303194838403986No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA277240.14278595883135328No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT263080.13549318297991783No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA240690.12396173867810713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC218100.11232728906766032TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT200600.10331432456200211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)