FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22260676
Sequences flagged as poor quality0
Sequence length125
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT311230.13981156726776853No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA278090.12492432844357468No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC269070.12087234008526963No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA232410.10440383751149336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC60050.0106.371841
CGGTTAA424850.0101.023571
CGGCTAA4250.0100.945851
CGGGCGC152900.099.5699541
GGGAGGC508000.095.289222
CGGGTAC63050.094.5060961
CGGGAGG1140700.094.2449651
CGGGTGT254800.089.776691
CGGGTAT275300.085.320871
CGGTAAT117750.084.30611
CGGGTTT576250.084.108161
CGGAATA208700.083.854511
CGGGTAA96350.083.241181
CGGGAAG178150.083.116151
GCGGAGT331950.083.0257957
GGGCGCG200500.080.1506042
CGGAGTG101200.079.0163351
GGAGGCG612650.078.63983
GGCGGAG601350.077.921446
CGGTATT277100.077.1974261
Produced by FastQC (version 0.11.5)