FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17895984
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC640380.3578344728068599No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT372990.2084210625132432No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA224680.12554772065062195No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT212880.11895406254274703TruSeq Adapter, Index 15 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT207130.11574105117662153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC292500.0114.5826951
CGGTTAA1253600.0113.7581561
CGGGTGC86250.0111.083361
CGGCTAA7950.0108.538321
CGGGTAC108850.0108.302451
GGGCGCG344800.0102.473762
GGCGCGG358650.095.1784063
CGCGGTG357100.094.975135
CGGAATA159800.093.545971
GCGCGGT361250.092.747774
CGGGTTT1007400.092.335151
CGGAGTG115200.091.79461
CGGGTAT349250.091.499791
CGGATAA88650.091.2269061
CGGGCGT468250.090.677271
CGGGTGT310350.090.504981
CGGAATT224450.090.357081
GCGGTGG519900.086.581986
CGGATGC12800.086.473931
CGGGTAA87750.086.465981
Produced by FastQC (version 0.11.5)