FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18069315
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA652090.3608825237702702No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT557000.30825739658642287No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA436140.24137052234686263No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT334540.18514260225138585No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA324680.17968583756495474No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT271070.1500167549240245No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT258610.14312108677058316No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC244540.13533440531641625No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG238130.13178695484582564No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA205700.11383940121692494No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA195690.10829962286893553No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT192600.1065895414408349No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAA191440.1059475691247842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC70800.0109.787421
CGGCTAA4750.0105.251511
CGGTTAA406150.0103.706371
CGGGCAT2600.0100.721451
GGGAGGC517450.098.971352
CGGGCGC154500.098.540491
CGGGTAC56750.098.4787141
CGGGCTT5000.097.6082461
CGGGAGG1047600.096.581981
CGGTCTA1050.096.361221
GCGGAGT366400.092.886687
GGGTGGC74600.091.7457662
CGGGTGT244550.090.7299961
CGGGTAA92550.090.481611
GGCGGAG652350.088.134636
CGGGGAC21000.087.858761
CGGAATA175450.087.554261
CGGATGC7750.087.547921
CGGATTG97600.087.446411
CGGGTTT461950.085.6781
Produced by FastQC (version 0.11.5)