FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21126477
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC540700.255934768489796No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT308940.14623356274687918No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT249170.11794204968485754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC275350.0113.239891
CGGGTGC78500.0112.912261
CGGTTAA1087900.0112.108281
CGGCTAA11050.0104.110561
CGGGTAC103700.0103.752411
GGGCGCG328850.0100.631922
GGCGCGG341500.093.939253
CGCGGTG347450.092.193415
CGGATAC14050.091.6383361
GCGCGGT343500.091.346184
CGGGCGT410000.091.1992341
CGGGTAT313850.090.365381
CGGGTGT268900.089.9097751
CGGAATA173550.087.9942551
CGGGTTT808400.087.464411
CGGAAAT104550.087.458311
CGGATGC11800.086.3801351
CGGATAA90450.085.407541
CGGTAAT114600.084.6257861
CGGTGGT608000.084.574357
Produced by FastQC (version 0.11.5)