FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20240078
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT710220.3508978572118151TruSeq Adapter, Index 15 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC612670.3027014026329345No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT344870.1703896595655412No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA344840.1703748374882745No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT319420.15781559735095882No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA263420.13014771978645537No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA248260.1226576300743505No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG230850.11405588456724328No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT210080.10379406640626582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC309100.0114.209071
CGGTTAA1134750.0112.965551
CGGGTGC114350.0112.2521
CGGGTAC116800.0107.1474151
CGGCTAA9200.0104.738411
GGGCGCG376050.0102.032322
CGGGCAT4950.097.3326641
GGCGCGG390550.095.367373
CGCGGTG394200.094.875655
CGGAATA226050.093.833011
GCGCGGT389850.093.7529144
CGGGTGT372050.093.55821
CGGGTAT384600.091.711591
CGGGTTT1000750.091.133991
CGGGTAA116950.091.090741
CGGGCGT469500.088.6958541
CGGGGAC30050.088.6772841
CGGTGGT740200.088.55847
GCGGTGG601950.088.4188466
CGGAGTG120250.088.343611
Produced by FastQC (version 0.11.5)