FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005418790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37390091
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA761330.20361811903586968No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC742220.19850713923108668No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT706020.1888254297107755No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG675250.18059597661851104No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT578640.15475758002300663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC537030.14362896308543352TruSeq Adapter, Index 7 (100% over 50bp)
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA526260.14074852077787134No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA487930.1304971416089894No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA483600.12933908077410136No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT466430.12474695501543444No Hit
TGGTTTTATGAAAGGAAGTGTTTAATTTTATTGAGTTGAATGTAAATATT431940.11552258591721533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)