FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18609419
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC706070.3794153917432887No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC346910.18641635184849134TruSeq Adapter, Index 12 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT269640.14489436773926148No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA231850.12458744681926932No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT198820.10683837039727033No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT188290.10117994548889463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC364950.0115.039861
CGGGTGC118900.0114.3153151
CGGTTAA998700.0112.362621
CGGGTAC143600.0108.612121
GGGCGCG434750.0103.0726552
CGGCTAA7150.0102.3290941
CGGGTAT405400.096.224951
CGGGCAT2600.096.0895161
GGCGCGG451100.095.973293
CGCGGTG449450.095.611425
CGGGTGT352050.095.329821
CGGATAC19350.095.297341
GCGCGGT449600.094.482124
CGGGCGT517400.093.985611
CGGATGC13400.091.4455261
CGGAATA156750.091.417491
CGGGTTT883850.090.93711
CGGAAAT107400.089.669061
CGGTAAT131800.089.3613741
CGGTGGT804900.089.311027
Produced by FastQC (version 0.11.5)