FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13357196
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT700530.5244588759497129No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA485010.3631076462455144No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC433150.32428213226788016No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT378460.2833379101422185No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA284560.21303872459459303No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT195230.14616091580897667No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT187600.14044863907065525No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA163030.12205405984908807No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT162140.12138775233963776No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA157480.1178989961665607No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT157240.11771931773704601TruSeq Adapter, Index 16 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA144620.10827122698506483No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT140020.10482739041936645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC236050.0113.146871
CGGGTGC82100.0111.581991
CGGTTAA552450.0111.1487351
CGGGTAC94500.0109.4835741
GGGCGCG280050.0100.741542
CGGGTAT294600.096.603831
CGGGTGT251650.096.168051
CGCGGTG285550.093.7134555
CGGGAGG958700.093.616811
GGCGCGG290200.093.196083
CGGGTTT549750.093.048151
CGGAATA204350.092.98161
GGGAGGC404950.092.8437042
GCGCGGT286350.092.474944
CGGGCGT352300.091.551771
CGGATAC10750.090.315041
CGGTAAT83650.089.3632051
CGGCTAA4000.089.3454061
CGGGTAA74900.088.828491
CGGAATG1284450.088.330991
Produced by FastQC (version 0.11.5)