FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20480801
Sequences flagged as poor quality0
Sequence length125
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT672550.32838071128175117No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA543170.2652093538724389No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA475350.23209541462758218No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT410990.20067086243355423No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC396970.19382542704262398No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA277560.13552204330289622No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT266660.1301999858306323No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT261440.12765125738978667No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA253240.12364750773175327No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA214570.10476641025905188No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA206330.10074313011488173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA10500.0111.665391
CGGTTAA745550.0110.117191
CGGGTGC98450.0109.724241
CGGGCGC240150.0104.982051
CGGGTAC92300.0102.6556851
CGGATTG190000.0100.3019561
CGGAATA368350.096.46181
GGGAGGC470050.095.3670652
CGGTACT509.094947E-1295.227331
CGGGAGG1026050.094.979081
CGGATGA74800.093.4131851
CGGGTGT281950.093.196641
CGGGTAA103750.092.072231
GGGCGCG302850.091.951252
CGGTAAT130150.091.7336041
CGGTCAA5450.091.732761
CGGGGAC25800.090.659751
CGGAGTA128700.090.0849151
CGGGCAT2850.089.7977141
CGGGTAT288800.089.625981
Produced by FastQC (version 0.11.5)