FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19647815
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA274830.13987814930057108No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG252320.12842140461929227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA797300.0108.290441
CGGGTGC86650.0105.425131
CGGGCGC151100.093.383771
CGGGTAC71500.091.889821
CGGGTTT921650.088.049181
CGGGTGT297350.088.042091
CGGATAA72550.087.852961
CGGTAAT114800.086.313091
CGGAATA134400.084.131511
CGGAATT189750.084.05381
CGGGTAT289450.083.290031
CGGGATA107950.083.0245741
CGGTATT333600.082.988521
CGGAAAT90200.081.482581
CGGGAGG683400.080.812281
CGGATGA67400.080.703181
GGGAGGC234400.080.640532
CGGGCGT334650.080.025061
CGGGTAA76250.079.219211
CGGAGTG95850.078.8525851
Produced by FastQC (version 0.11.5)