FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18384605
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC638100.34708387805993113No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT351130.19099132127124843No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT229020.12457161848187656TruSeq Adapter, Index 15 (97% over 40bp)
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA215880.11742433410997953No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT215410.1171686854300106No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT194160.10561010149524562No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA186970.10169922062508278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC334400.0114.498281
CGGTTAA1186950.0113.623541
CGGGTGC95800.0112.523151
CGGGTAC121500.0108.6011661
CGGCTAA6400.0107.826931
GGGCGCG399750.0102.126982
GGCGCGG415950.095.065443
CGCGGTG415550.094.7125245
GCGCGGT413600.093.7214364
CGGAATA146050.092.708581
CGGGTGT303950.092.675951
CGGGCGT467200.091.871911
CGGGTAT349550.091.563451
CGGATAC19050.089.6264341
CGGTAAT114500.088.430741
CGGGTTT879000.088.3494641
CGGTGGT718950.088.3436367
GCGGTGG598400.086.920426
CGGAGTG103750.086.870781
CGGAAAT94250.086.600811
Produced by FastQC (version 0.11.5)