FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21553442
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC620760.28800968309377223No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT423830.19664144594631333No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT245130.11373125461817189No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT241780.11217697850765553No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA225430.10459118316229955No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT222510.10323641114955097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1555150.0114.689661
CGGGCGC343350.0114.4218441
CGGGTGC105350.0112.090141
CGGCTAA10450.0110.203961
CGGGTAC131200.0109.1979451
GGGCGCG410350.0102.262562
GGCGCGG418200.097.16363
CGGAATA217400.095.351251
CGCGGTG423200.095.3111955
GCGCGGT425300.093.2704244
CGGGTAT400150.092.453851
CGGGTGT350350.091.8170551
CGGGTTT1074250.090.761741
CGGATAC18900.089.978591
CGGGCGT510650.089.963661
CGGTGGT757650.088.4126057
CGGAGTG120550.088.00771
CGGTAAT125550.087.82971
CGGATAA103600.087.3739551
GCGGTGG625650.087.250186
Produced by FastQC (version 0.11.5)