FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18838303
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC352530.18713469042301742No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT287060.15238102922540314No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA240920.1278883772068004No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA220750.11718146799103932No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA206450.10959055069875456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC99500.0111.8411561
CGGATCC1600.0111.57951
CGGCTAA5600.0110.516821
CGGGTCT10650.0107.842711
CGGGCGC205800.0106.960161
CGGTTAA590000.0106.6825941
CGGGTCC4950.0104.591691
CGGGTAC98600.0104.533161
GTTCCCC401.6007107E-10104.134783
GGTTCCA1600.0104.1342242
GGTGCCT1500.0103.142472
CGGTACT2150.0102.410951
GGGTCCT2850.0102.3073042
CGGTACC1050.0102.015541
CGGGTCA4250.0100.815351
CGGTTCA7750.099.8216551
CGGTTCT7050.098.759731
CGGTTCC6300.098.237181
CGGATCT3150.098.237181
CGGGGAC41750.098.065241
Produced by FastQC (version 0.11.5)