FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13446814
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT593260.44119000976736944No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT391450.29110984951528296No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA332010.24690607009214227No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC316190.23514120147716777No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT285590.21238488165300717No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT246350.18320324799614243No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA244690.18196875482921085No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA214690.1596586373545436No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT188930.14050168314962935No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA181210.134760546252815No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA143750.1069026462327805No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA141560.10527400765712978No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT140620.10457495730959022No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA139740.10392052719699997No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA137210.10203904062330305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC68650.0110.536991
CGGTTAA497300.0109.746031
CGGGCGC187250.0106.732541
CGGGTAC76700.0104.4451
CGGCTAA2900.0100.56251
TAAGTCC600.099.19431
GGGAGGC430350.096.3524862
CGGGAGG954250.095.363751
CGGGTGT230250.094.1408841
GGGCGCG230400.093.317282
CGGGTAT252700.092.3719941
CGGAATA147450.091.787531
CGGGTTT487650.090.730291
CGGTAAT81350.090.573491
CGGGTAA71050.089.882191
CGGATGC6300.088.802521
GCGGAGT286450.087.819647
CGGGCGT333300.086.748141
CGCGGTG236600.086.409555
GGCGCGG239800.086.3034063
Produced by FastQC (version 0.11.5)