FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17986409
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC581510.32330522451702287No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC389750.21669139181701028TruSeq Adapter, Index 12 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT293120.16296749395613097No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT232370.12919199157541675No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT188390.10474019577782313No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT186100.10346701223129086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC299550.0114.019351
CGGTTAA1062800.0113.677871
CGGGTGC95900.0112.160551
CGGGTAC118650.0107.301641
CGGCTAA7900.0103.923161
GGGCGCG363300.0100.3077552
CGCGGTG366100.096.04735
CGGAATA138950.094.365531
GGCGCGG372800.094.321133
GCGCGGT367550.093.807454
CGGGTAT348550.093.706051
CGGGTGT308500.093.413421
CGGGCGT437800.091.928961
CGGATAC15400.090.7836151
CGGGCAT2500.090.42821
CGGGTTT833400.090.280581
CGGTGGT655950.089.239487
CGGATGC11850.088.359791
GCGGTGG539550.088.010746
CGGTAAT110050.087.63011
Produced by FastQC (version 0.11.5)