FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21357375
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC820240.38405468836877193No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT470970.2205186732920127No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA274150.12836315324331757No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT272960.1278059686642202No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT265110.12413042333151898No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT260610.12202342282232718TruSeq Adapter, Index 18 (97% over 40bp)
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG240770.11273389168846826No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA219240.102653064807824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC375850.0114.610681
CGGTTAA1574250.0113.8149641
CGGGTGC118200.0111.6279141
CGGCTAA12500.0106.933611
CGGGTAC136000.0106.191021
GGGCGCG441600.0101.522812
GGCGCGG458900.095.020853
CGCGGTG465600.094.25315
CGGAATA233550.093.709031
GCGCGGT461850.092.909814
CGGGTAT416950.091.772181
CGGGTGT366500.091.5022351
CGGGCGT556800.090.0075761
CGGATGC13850.089.647511
CGGTAAT136600.089.546241
CGGGTTT1083350.089.477391
CGGAGTG132250.088.942891
CGGATAA120400.088.519241
GCGGTGG668050.088.336596
CGGAATT270200.088.1659241
Produced by FastQC (version 0.11.5)