FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005418830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51992133
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2225540.42805322105173105TruSeq Adapter, Index 18 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1041250.20027068325894612No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT821060.15792004532685744No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT727730.13996925265597393No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA618750.1190083892114986No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA607290.11680420959070865No Hit
TGGAGTAGTAGGTTATAATGTGGGAGATTATTTTGAAGTTTGGTAGGATA561000.10790093955175874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)