FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20105314
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC625910.3113157048927463No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT376620.18732361006647297No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT273030.13579991836983993No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT261170.1299009804074684No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC251480.1250813590874532TruSeq Adapter, Index 2 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA233820.11629761166624902No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT210400.10464895002385936No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT204500.10171440247090893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1264200.0113.013471
CGGGCGC336800.0112.8923651
CGGGTGC108250.0111.749411
CGGCTAA9850.0107.52751
CGGGTAC127550.0104.077031
GGGCGCG416200.096.883682
CGGGTGT328050.091.8521651
CGCGGTG422600.091.324095
GGCGCGG427900.091.2870253
CGGGTAT351100.090.940151
CGGAATA202950.090.184731
CGGGCGT480400.090.083731
GCGCGGT428250.089.1760254
CGGGTTT970900.087.8289641
CGGTGGT742450.086.67417
CGGGTAA103850.086.517951
CGGTAAT112750.085.810371
GCGGTGG617300.085.2421956
CGGATAC19350.085.1794051
CGGAGTG116850.084.428991
Produced by FastQC (version 0.11.5)