FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17251891
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT604790.35056446855593976No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA430020.24925963188615088No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA391650.22701859175901354No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC377500.21881659233761677No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT369680.21428375590826537No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT361010.2092582198670279No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA255490.14809391040089462No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT231700.1343041177341081No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT202090.11714078184240789No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA198530.11507723993850877No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA198130.11484538129762122No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT196390.11383679620976042No Hit
CGGAATATTTTTATATAAAATTAAGATAGAAGTATTTTCGGAAATATTTT196100.11366869869511695No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA184130.1067303288665573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA10300.0112.671841
CGGGTGC81500.0111.871421
CGGTTAA686800.0111.142191
CGGGCGC212850.0106.8928451
CGGGTAC82800.0103.789841
CGGAATA293000.099.447021
CGGATGC9100.096.791751
CGGGAGG991250.095.45031
GGGAGGC424500.095.178262
CGGGTGT263200.094.833241
CGGTAAT119150.094.053351
CGGTACT950.093.969231
GGGCGCG266800.093.934142
CGGTCTA1400.093.521761
GCTAATT12550.092.9006653
CGGGTAT289300.091.852511
CGGAGTG111100.091.065291
CGGGTAA84350.091.017031
CGGGAAG153900.090.179521
CGGGTTT580300.089.388711
Produced by FastQC (version 0.11.5)