FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13381346
Sequences flagged as poor quality0
Sequence length125
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC407760.3047227087618839No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT293350.21922308861903728No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT259460.19389678736354324No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA237730.17765776327732652No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA215240.16085078436802994No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA167580.12523403848910267No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT160850.12020464906893523No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT152400.1138898882070608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC192000.0114.195131
CGGTTAA877450.0113.6767651
CGGGTGC53100.0112.060871
CGGGTAC72200.0105.680511
CGGCTAA7000.0105.513161
GGGCGCG230200.0101.422172
CGGAATA183100.096.193561
GGCGCGG239250.094.8262943
CGCGGTG244850.092.195925
CGGGTTT710500.091.043461
GCGCGGT245750.090.454384
CGGGTGT212350.089.759561
CGGATAA61950.089.417941
CGGGTAA63550.088.572571
CGGGTAT240000.088.5268251
CGGATGC8650.087.452171
CGGAATT170150.087.131651
CGGGCGT330750.087.03621
CGGAGTG84100.086.477411
CGGATAC11850.086.45561
Produced by FastQC (version 0.11.5)