FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19542211
Sequences flagged as poor quality0
Sequence length125
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT479290.24525883995418943No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA375610.1922044542452233No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA332490.1701393972258308No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC311330.15931155384618453No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT247180.12648517611441204No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT197730.10118097691197787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC76150.0110.905711
CGGTTAA584900.0108.5430761
CGGGCGC192400.0105.794341
CGGGTAC75900.0105.625131
CGGCTAA6850.099.919151
GGGAGGC415300.097.2075962
CGGAATA289950.096.967951
CGGGGAC23500.096.4936141
CGGGAGG929950.096.3012161
GGGCGCG234350.094.373722
CGGGTGT226350.090.136721
CGGATGC8400.089.984121
GCGGAGT284250.089.613747
CGGGAAG138800.089.061181
CGGAGTG103100.088.842631
CGGGTAT230050.088.273061
CGGATTG100900.086.886641
CGGGTTT467350.086.80181
CGGTAAT106450.086.717661
GGCGCGG249500.085.868933
Produced by FastQC (version 0.11.5)