FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14137815
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC440230.31138475075533245No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT150400.10638136091043772No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT143570.1015503456510076No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT143530.10152205273587184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA626000.0110.2903061
CGGGTGC84150.0109.922861
CGGGCGC233150.0109.371751
CGGGTAC95200.0105.72991
CGGCTAA5800.098.522261
GGGCGCG283050.096.0469062
GGGAGGC440950.095.732872
CGGTAAT100000.093.928661
CGGGTAT303450.092.801911
CGGGAGG1021000.092.6321951
CGGGTGT277500.092.449681
CGGGTTT624150.090.99051
CGGGTAA84150.089.9754641
CGGAATA115550.089.324431
CGGATAC12950.089.170891
CGCGGTG291300.088.466545
GGCGCGG296850.088.215653
GCGCGGT290450.088.1108554
CGGGAAG143250.085.930441
CGGAGTG84150.085.589871
Produced by FastQC (version 0.11.5)