FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22929299
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC863530.37660549500444823No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT449710.19612897891034525No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT341230.14881833064325256No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA279900.12207089279092222No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT262490.11447798731221569No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC249070.10862521353138618TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC424950.0114.9889451
CGGTTAA1518350.0113.5130541
CGGGTGC128100.0113.4059141
CGGGTAC159900.0111.091421
CGGCTAA11250.0111.046781
GGGCGCG503450.0103.278042
GGCGCGG521250.096.680013
CGCGGTG528000.095.116385
CGGGTAT451050.094.486571
GCGCGGT524200.094.4558644
CGGAATA242350.093.965441
CGGGTGT405100.093.64691
CGGGCAT3750.093.596571
CGGGCGT606800.092.008491
CGGGTTT1117900.090.237141
CGGTAAT153000.090.0894241
CGGTGGT925250.089.01837
CGGATAC20600.088.945241
GCGGTGG753050.088.707736
CGGATAA117700.087.692451
Produced by FastQC (version 0.11.5)