FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19374889
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA527700.2723628506981382No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT491890.25388016416506953No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA483480.2495394941359406No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA449160.23182584426677233No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC345340.17824102114855986No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT339010.17497390565695628No Hit
CGGGGACGCGGAAGTTTGTTTGAGGGAGGAGGGGTGGAAGGAGAGATAGT271650.14020725486478916No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG258260.1332962475294697No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC255190.13171172232264144TruSeq Adapter, Index 5 (100% over 50bp)
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT230720.11908197254704272No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT219590.11333742350730372No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA217240.11212451333269574No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA201090.10378898170719843No Hit
CGGTACGAGATCGATAGTTAATAAGTATCGTAAGGGAAAGTTGAAAAGAA198130.10226123101918158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA6800.0106.987831
CGGGTGC79150.0105.1763151
CGGTTAA547900.0103.6250461
CGGGCGC204150.099.811151
CGGAATA321850.096.753541
GGGAGGC523800.096.4336852
CGGGTAC84650.094.186341
CGGGAGG1225200.093.2160341
CGGTAAT123350.091.660591
CGGGTGT291850.090.026071
CGGGTAA103000.088.927791
GGGACGC46050.086.552693
CGGAGTG131800.086.2817151
CGGGTAT302800.086.238771
GCGGAGT346850.085.447117
CGCGGAA46450.085.423147
CGGGTTT673600.084.739141
CGGGGAC53000.084.048161
CGGGAAG189050.083.7789461
CGGTATT304250.083.5541841
Produced by FastQC (version 0.11.5)