FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19364065
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC562130.29029545191053635No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA316790.16359684807916108No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC300490.15517919403802868TruSeq Adapter, Index 7 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT227720.11759927473905918No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG226510.11697440594214076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA969850.0111.611241
CGGGCGC346800.0111.174931
CGGGTGC105350.0111.054091
CGGGTAC131700.0107.706021
GGGCGCG406750.0100.774072
CGCGGTG413200.094.540795
GGCGCGG419850.094.3483053
CGGGTAT433550.093.9611051
CGGGCGT560750.093.957571
GCGCGGT413200.093.749724
CGGGTGT371000.091.328291
CGGGTTT1015650.090.898381
CGGTGGT743900.086.871577
CGGAATT206700.086.329671
GCGGTGG612800.086.082746
CGGTAAT117900.085.988291
CGGAAAT97650.085.082321
CGGAATA139250.083.675891
CGGATAA78900.082.1111
GGGCGTG385800.081.827782
Produced by FastQC (version 0.11.5)