FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17037608
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC354770.20822758687721893No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA245760.14424560067352177No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG200580.11772779371376546No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT194920.11440573113314968No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT182730.10725097091094009TruSeq Adapter, Index 15 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA899700.0112.0489041
CGGGCGC242850.0109.182451
CGGGTGC88250.0108.7834551
CGGGTAC101050.0104.898881
GGGCGCG292400.097.273812
CGGGTAT342050.091.994731
CGGGTGT312800.091.026461
CGGGTTT909350.090.5375061
CGGGCGT406850.090.2597351
CGCGGTG303000.089.880095
GGCGCGG308000.089.468963
CGGAATA132750.088.278391
GCGCGGT306400.087.878044
CGGAAAT86150.087.876971
CGGTAAT107900.085.718151
CGGAATT178300.085.320371
CGGTGGT568700.083.082597
CGGATAA67550.082.8221
CGGAGTG95350.082.456591
CGGGATA94600.082.1036761
Produced by FastQC (version 0.11.5)