FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005418962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23446500
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC553060.23588168809843685No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT375120.15998976393065062No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT363720.15512763098969998TruSeq Adapter, Index 13 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT337350.14388074979207985No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA307870.13130744460793722No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG294010.12539611455867614No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA287430.12258972554539058No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA267300.11400422237860662No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA255890.10913782440876037No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT253470.10810568741603224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)