FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005418965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25195599
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC863080.34255188773245676No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC560210.22234438641446866TruSeq Adapter, Index 4 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT534810.21226326073851232No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA412280.16363175171981423No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG370330.1469820185660202No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT351590.13954421166966502No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT318520.12641890355533916No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA302140.11991776817848229No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA285180.11318643386886734No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA281550.11174570606557122No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA257360.10214482299071358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)