FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19370313
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC854800.44129385002710075No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT248310.12819101064603344No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT238960.12336403650266262No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT215440.11122174432596932No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA210200.10851657379000534No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA203820.1052228737862935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC416200.0114.324911
CGGGTGC127250.0113.1880341
CGGTTAA900500.0110.751891
CGGGTAC165900.0108.996251
GGGCGCG481800.0103.503742
CGGCTAA7300.0100.6403661
CGGATAC19450.098.391211
CGGGTAT491650.097.2984851
GGCGCGG497700.096.4656453
CGCGGTG493750.096.1175165
GCGCGGT489150.095.910914
CGGGTGT408000.093.722131
CGGGCGT653400.093.704991
CGGAATA195650.092.8162461
CGGTAAT158800.091.520931
CGGATGC12850.091.292571
GGGAGGC458450.089.610192
CGGGCAT4300.089.560861
CGGGTTT883250.089.4773251
GCGGTGG705250.089.179466
Produced by FastQC (version 0.11.5)