FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005418995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005418995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20052855
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC886000.4418323475634766No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT524120.26136926637129726No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA396410.19768257437656633No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT302880.15104083682847155No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA286900.14307189674487747No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA231650.11551971028564262No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT220030.10972502419231576No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT218290.10885731732464031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC437900.0114.32681
CGGGTGC135650.0113.387511
CGGTTAA859250.0110.314251
CGGGTAC172050.0109.986661
CGGCTAA8450.0107.772681
GGGCGCG497300.0104.808652
GGCGCGG513550.098.245043
CGGGTAT527550.098.01471
GCGCGGT506200.097.7444
CGCGGTG512350.097.153325
CGGGTGT433450.096.305011
CGGGTTT879350.093.280461
CGGGCGT672050.092.5323641
CGGAATA254100.092.2980961
CGGATAC20050.092.020571
CGGTAAT156200.091.9204941
GGGAGGC534350.091.443842
GCGGTGG728900.091.2534946
CGGGTAA121350.090.347181
CGGTGGT906150.090.206357
Produced by FastQC (version 0.11.5)