FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18676841
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC464790.24885900136966416No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT215580.115426372157904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA973050.0112.321181
CGGGCGC304450.0111.6431961
CGGGTGC100450.0110.359031
CGGGTAC121800.0108.7771
GGGCGCG362250.0102.033522
GGCGCGG371200.096.169583
GCGCGGT368800.094.730544
CGGGTAT375650.094.258611
CGGGCGT462100.094.2169651
CGCGGTG376100.093.840845
CGGGTGT328550.092.841831
CGGAAAT99250.091.216241
CGGAATT208300.090.444071
CGGGTTT887400.090.2258151
CGGAATA164150.089.572181
CGGTAAT124600.089.4953841
CGGATAA84650.087.586721
CGGTGGT691750.087.504947
GCGGTGG561350.086.7573246
CGGAGTG104350.086.358321
Produced by FastQC (version 0.11.5)