FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16213661
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC585520.36112757013977287No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA530380.3271192113860034No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG498180.3072594153781802No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA429860.26512210906592903No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT266370.1642873870373878No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT213280.13154339417852637No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTT166940.10296255731509373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC105850.0115.263791
CGGGCGC306350.0114.119591
CGGTTAA1013700.0113.8276441
CGGGTAC122000.0110.867161
CGGCTAA6600.0108.5275961
GGGCGCG358750.0103.760622
CGGAATA173150.099.489371
CGGGTGT286000.096.923491
CGGGTAT332950.096.827351
CGGATGC11500.096.5423661
GGCGCGG375850.095.905263
CGGGCGT405850.095.863141
CGCGGTG377800.094.843275
CGGTAAT123250.094.24511
GCGCGGT377000.093.845034
CGGAATT198650.092.0968551
CGGGTTT657750.091.5566941
CGGGTAA83200.090.683151
CGGGAGG774750.090.665891
CGGATAA89250.089.81951
Produced by FastQC (version 0.11.5)