FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22024646
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT977260.44371201244278796No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA722510.32804613522505655No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC558880.25375209208810895No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT554760.25188146043300763No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA533290.24213329013324436No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT321210.145841163576477No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT320640.14558236259506738No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA300020.1362201235833711No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT257790.11704614911858288No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA240510.10920039305058524No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA232770.10568614814512796No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA228710.1038427586985961No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA228550.10377011280907761No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA224960.10214012066300635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA8850.0113.656531
CGGGTGC105900.0113.3602751
CGGTTAA865800.0111.660511
CGGGCGC304750.0109.662211
CGGGTAC113000.0105.8689351
GGGAGGC636300.098.68972
CGGAATA400250.097.0732961
CGGGAGG1453700.095.900011
GGGCGCG378600.095.076082
CGGGTGT338250.094.3847661
CGGTAAT152250.093.235661
CGGGTAT379750.092.7373661
CGGATTG174850.092.451741
CGGGTAA116350.090.594771
CGGATAC15750.089.938851
CGGGGAC34650.089.835791
CGGAAAT122900.089.786031
GCGGAGT418400.089.6039357
CGGGCGT490800.089.010751
CGGAGAA156550.088.621711
Produced by FastQC (version 0.11.5)