FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21075484
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC646290.3066548791951824No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT518020.24579269448806018No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA404090.1917346239830127No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT287940.1366231968860122No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA268020.127171456655515No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT212370.10076636911399046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC131150.0114.123511
CGGGCGC342900.0111.858591
CGGTTAA870800.0110.6310041
CGGGTAC132450.0108.461681
CGGCTAA9550.0104.151141
GGGCGCG422500.099.049642
CGGGTGT390000.095.921321
CGGGTAT393900.094.8959961
CGGATGC15450.092.519591
GGCGCGG438300.092.344823
CGGAATA242200.092.142611
CGCGGTG435950.091.7513055
CGGGCGT511800.091.3175661
GCGCGGT438500.090.336214
CGGGTAA112000.090.243261
GGGAGGC502400.090.2336042
CGGAAAT118450.089.904911
CGGGAGG1132150.089.706171
CGGGTTT772500.089.682331
CGGGGAC35800.088.8401261
Produced by FastQC (version 0.11.5)