FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18333324
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC681880.3719347348031377No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT380200.20738192375807027TruSeq Adapter, Index 18 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT290750.15859098982814027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC344900.0115.150741
CGGGTGC115700.0114.679181
CGGTTAA1055450.0112.9152151
CGGGTAC135450.0108.7243041
CGGCTAA6700.0103.478031
GGGCGCG401700.0103.28782
CGGGTAT418600.096.2047961
GGCGCGG417450.096.011013
CGGGTGT356200.095.389711
CGCGGTG419350.095.107575
GCGCGGT416100.094.434124
CGGGTTT996400.092.818351
CGGGCGT523350.092.6290361
CGGAATA154000.091.8245851
CGGTAAT128050.091.063141
CGGAATT222700.088.295931
CGGATAA83900.088.2621151
CGGATGC12650.087.879461
GCGGTGG623700.087.2649466
CGGTGGT774200.087.1922457
Produced by FastQC (version 0.11.5)