FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005419041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005419041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17316888
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC578890.3342921661213031No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT506720.29261608667792965No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA403930.23325784632897087No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT312300.180344181933844No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA253070.14614057675951939No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT219330.12665670644748642No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG196920.11371558215309818No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA189460.10940764876460482No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT181790.1049784464737544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC105850.0116.1866151
CGGGCGC297650.0114.057691
CGGTTAA840800.0113.959131
CGGGTAC126650.0111.654031
CGGCTAA6250.0108.618281
GGGCGCG356200.0100.217692
CGGGTAT372600.097.881821
CGGGTGT318000.096.675631
GGGAGGC520850.095.961062
CGGGAGG1187250.095.586861
CGGAATA210250.094.097711
CGGGTTT721750.093.932111
CGGGCGT477750.093.605821
GGCGCGG370600.093.492883
CGGATGC12450.093.475281
CGCGGTG372450.092.48655
GCGCGGT366850.092.454734
CGGGTAA97400.092.412861
CGGTAAT118600.092.401421
CGGATAC14800.091.738411
Produced by FastQC (version 0.11.5)